Technology
The complete removal of all xenogenic cells significantly reduces immunogenicity, ensuring a high level of biocompatibility and safety for patients. This process forms the foundation of our commitment to delivering implants with optimal biological performance and minimal immune response.
Native Tissue
Native tissue consists of both cellular elements and the extracellular matrix (ECM). The ECM is produced by cells and surrounds them, providing structural and biochemical support. It is composed primarily of proteins such as collagen, elastin, and proteoglycans, which together define the mechanical strength, elasticity, and resilience of the tissue.
In contrast, the cells and cellular components within native tissue are the primary contributors to immune rejection when used in xenogenic implants. Their removal is essential to reduce immunogenicity and improve the safety and acceptance of biological grafts.
Decellularisation Process
To prevent immune rejection and implant degeneration, Auto Tissue Berlin uses the naturally occurring detergent deoxycholic acid (DOA) during processing. This step effectively removes all cells and cellular components from the tissue, ensuring a fully decellularised matrix with significantly reduced immunogenic potential.
Decellularised Tissue
Decellularised tissue is defined by the absence of cell nuclei and an extremely low residual DNA and RNA content. This high level of purification contributes to the viral safety of the implants, as the minimal presence of nucleic acids significantly reduces the risk of pathogen transmission. As a result, decellularised implants offer a safe and biocompatible alternative to autologous tissue.
Structure
Decellularised tissue is characterised by the absence of cell nuclei and very low levels of residual DNA and RNA. Our implants are composed entirely of extracellular matrix (ECM), ensuring a structurally intact, biocompatible scaffold that supports tissue integration while minimising immunogenic response.
Mechanical Stability
The mechanical properties of the decellularised tissue are preserved, providing the implant with stability comparable to native or glutaraldehyde-treated tissue. This demonstrates that cells and cellular components are not necessary for maintaining the structural integrity and mechanical strength of the tissue.
Removal of DNA and RNA
Our implants undergo a liquid sterilisation process that ensures effective sterilisation without compromising the integrity of the extracellular matrix (ECM). During this process, DNA and RNA are completely removed, further enhancing the safety and biocompatibility of the final implant.